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产品目录

SmiI内切酶图片
产品货号:
GS2167
中文名称:
SmiI内切酶
英文名称:
SmiI
产品规格:
1kU
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)

使用说明

To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?

技术规格

Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA(SspI-digest)in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sitesATTT↑AAAT
来源Streptococcus milleri S
Assayed onT7 DNA(SspI-digest)
Optimal bufferO?(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.)+?BSA
Optimal temperature37℃
Storage conditions10 mM Tris-HCl(pH 7.5);250mMNaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;100μg/ml BSA;50%glycerol.Store at -20℃.
LigationsAfter 5-fold overdigestion with enzyme 80%of the DNA fragments can be ligated and recut.Ligation>95%in presence of 10%PEG.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of T7 DNA with 30 u.a.of enzyme for 16hoursat 37℃.
Methlation effectMethylation sensitivity not tested
Thermal inactivationenzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible EndsSwaI

相关搜索:SmiI内切酶SmiI限制性内切酶
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