产品货号：
GS2167
中文名称：
SmiI内切酶
英文名称：
SmiI
产品规格：
1kU
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?
技术规格
相关搜索：SmiI内切酶，SmiI限制性内切酶
使用说明
To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA(SspI-digest)in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | ATTT↑AAAT |
| 来源 | Streptococcus milleri S |
| Assayed on | T7 DNA(SspI-digest) |
| Optimal buffer | O?(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.)+?BSA |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);250mMNaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;100μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 5-fold overdigestion with enzyme 80%of the DNA fragments can be ligated and recut.Ligation>95%in presence of 10%PEG. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 30 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | SwaI |
相关搜索：SmiI内切酶，SmiI限制性内切酶